ICS11.220B41DB21辽宁省地方标准 DB21/T 2592.3—2016 鸡传染性疾病检测方法 第3部分：鸡马立克氏病毒实时荧光PCR检测方法 Detection method of chicken infectious disease Part3:The testing method of real-time fluorescent PCR for chicken Marek's disease 2016-03-18发布2016-05-18实施辽宁省质量技术监督局发 布DB21/T 2592.3—2016目次前言 ................................................................................ II1范围 .............................................................................. 12规范性引用文件 .................................................................... 13缩略语 ............................................................................ 14仪器与器材 ........................................................................ 14.1仪器 .......................................................................... 14.2器材 .......................................................................... 15试剂和材料 ........................................................................ 26操作步骤 .......................................................................... 26.1样品的采集、存放和运送 ........................................................ 26.1.1采样工具 .................................................................. 26.1.2样品的采集 ................................................................ 26.1.3存放与运送 ................................................................ 26.2实时荧光PCR检测.............................................................. 36.2.1DNA提取...................................................................36.2.2扩增体系的配制 ............................................................ 36.2.3加样 ...................................................................... 36.2.4荧光PCR扩增检测.......................................................... 37结果判定 .......................................................................... 37.1阈值设定 ...................................................................... 37.2质控标准