ICS11.220B41DB21辽宁省地方标准 DB21/T 2592.1—2016 鸡传染性疾病检测方法 第1部分：鸡传染性法氏囊病毒实时荧光RT-PCR检测方法 Testing method of chicken infectious disease Part1：The testing method of real-time fluorescent RT-PCR for chicken infectious bursal disease virus 2016-03-18发布2016-05-18实施辽宁省质量技术监督局发 布DB21/T 2592.1—2016目次前言 ................................................................................. 2第1部分：鸡传染性法氏囊病毒实时荧光RT-PCR检测方法 .................................. 31范围 .............................................................................. 32规范性引用文件 .................................................................... 33缩略语 ............................................................................ 34材料与试剂 ........................................................................ 34.1材料 .......................................................................... 34.2试剂 .......................................................................... 35采样 .............................................................................. 45.1采样工具 ...................................................................... 45.2样品采集 ...................................................................... 45.2.1活禽 ...................................................................... 45.2.2病死禽或禽类产品 .......................................................... 45.3样品贮运 ...................................................................... 45.4样品制备 ...................................................................... 45.4.1咽喉、泄殖腔拭子 .......................................................... 45.4.2肌肉或组织脏器 ............................................................ 55.5样品存放 ...................................................................... 56RT-PCR操作方法....................................................................56.1实验室分区设置与管理 ..........................................................