ICS11.220B41DB21辽宁省地方标准 DB21/T 2592.2—2016 鸡传染性疾病检测方法第2部分：鸡传染性支气管炎病毒荧光RT-PCR诊断技术 Detection method of chicken infectious disease Part2：Diagnostic technique of fluorescent RT-PCR for avian infectious bronchitis virus 2016-05-18实施2016-03-18发布辽宁省质量技术监督局发 布DB21/T 2592.2-2016目次前言 ................................................................................. 21范围 .............................................................................. 32规范性引用文件 .................................................................... 33缩略语 ............................................................................ 34仪器与器材 ........................................................................ 35试剂与材料 ........................................................................ 46操作步骤 .......................................................................... 46.1样品的采集、保存和制备 ........................................................ 46.1.1采样工具 .................................................................. 46.1.2样品的采集 ................................................................ 46.1.3样品的保存 ................................................................ 46.1.4样品的制备 ................................................................ 56.2核酸的提取 .................................................................... 56.2.1编号 ...................................................................... 56.2.2裂解 ...................................................................... 56.2.3沉淀 ...................................................................... 56.2.4洗涤 ...................................................................... 56.2.5干燥 ...................................................................... 56.2.6溶解 ...................................................................... 56.3RT-PCR检测....................................................................5